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KMID : 0381120170390060611
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Genes and Genomics
2017 Volume.39 No. 6 p.611 ~ p.621
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Genome-wide identification and resistance expression analysis of the NBS gene family in Triticum urartu
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Liu Jing
Qiao Linyi
Zhang Xiaojun
Li Xin
Zhan Haixian
Guo Huijuan
Zheng Jun
Chang Zhijian
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Abstract
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As the largest class of resistant genes, the nucleotide binding site (NBS) has been studied extensively at a genome-wide level in rice, sorghum, maize, barley and hexaploid wheat. However, no such comprehensive analysis has been conducted of the NBS gene family in Triticum urartu, the donor of the A genome to the common wheat. Using a bioinformatics method, 463 NBS genes were isolated from the whole genome of T. urartu, of which 461 had location information. The expansion pattern and evolution of the 461 NBS candidate proteins were analyzed, and 118 of them were duplicated. By calculating the lengths of the copies, it was inferred that the NBS resistance gene family of T. urartu has experienced at least two duplication events. Expression analysis based on RNA-seq data found that 6 genes were differentially expressed among Tu38, Tu138 and Tu158 in response to Blumeria graminis f.sp.tritici (Bgt). Following Bgt infection, the expression levels of these genes were up-regulated. These results provide critical references for further identification and analysis of NBS family genes with important functions.
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KEYWORD
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T. urartu, Computational biology, Genome-wide, NBS genes, Phylogenetic analysis, Resistance expression
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